Optimization of Ethanol Extraction Conditions of Antioxidant Compounds from Lotus Seeds Using Response Surface Methodology

doi:10.7506/spkx1002-6300-200918003

Abstract

Abstract:

Compounds having DPPH radical scavenging activity were extracted from lotus seeds using ethanol. On the basis of single-factor experiments, the effects of extraction time, ethanol concentration and temperature and interactions among them on DPPH radical scavenging activity of ethanol extract from lotus seeds using a three-factor, three-level Box-Behnken experimental design combining with response surface modeling (RSM) and a quadratic polynomial regression model describing the relationship of DPPH radical scavenging activity with the three variables was established. The optimum extraction conditions were determined as follows: ethanol concentration 100% and temperature 78 ℃ for an extraction duration of 6.0 h. Under the optimized conditions, the actual value of DPPH free radical scavenging activity was 76.17%, close to the predicted.

Key words: lotus-seeds, antioxidation, DPPH free radicals, response surface methodology

CLC Number:

R151.3

WANG Xi-xi，ZHENG Tie-song*，LI Qi-hong. Optimization of Ethanol Extraction Conditions of Antioxidant Compounds from Lotus Seeds Using Response Surface Methodology[J]. FOOD SCIENCE, 2009, 30(18 ): 34-38.

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Optimization of Ethanol Extraction Conditions of Antioxidant Compounds from Lotus Seeds Using Response Surface Methodology

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