FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (22): 140-145.doi: 10.7506/spkx1002-6300-200922030

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Extraction of Soybean Protein through Enzymatic Hydrolysis Combined with Pretreatment of Extrusion

LI Yang,JIANG Lian-zhou*,XU Jing,HU Shao-xin,YANG Liu   

  1. (College of Food Science, Northeast Agricultural University, Harbin 150030, China)
  • Received:2008-12-23 Online:2009-11-15 Published:2010-12-29
  • Contact: JIANG Lian-zhou*, E-mail:jlzname@163.com

Abstract:

Enzymatic hydrolysis combined with pretreatment of extrusion was used to extract soybean protein. An alkaline proteinase has been screened from 5 proteinases. The optimal condition for soybean protein extraction by alkaline proteinase was 1.9% proteinase, reaction at 50 ℃, 3.3 h of hydrolysis, 1:4.6 of material and water ratio, and pH 8.5. The total protein extraction rate reached to 93.76% under this optimal condition, which was nearly 15% higher than total protein extraction using enzymatic hydrolysis combined with traditional treatment of heating.

Key words: enzymatic hydrolysis method, extrusion, soybean protein

CLC Number: