Rapid Identification of Salmon Aquacultured with Synthetic Astaxanthin through High Performance Liquid Chromatography

doi:10.7506/spkx1002-6300-200922075

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (22): 318-320.doi: 10.7506/spkx1002-6300-200922075

Rapid Identification of Salmon Aquacultured with Synthetic Astaxanthin through High Performance Liquid Chromatography

MU Zhi-chun1，ZHANG Ming2，ZHANG Yi-bing1，GUAN En-ping1，LU Peng1，LENG Kai-liang3，DUAN Xiao-juan1，LIU Lin1，ZHENG Shi-xiang1

1. Shandong Entry-Exit Inspection and Quarantine Bureau, Qingdao 266002, China；
2. Qingdao Entry-Exit Inspection and Quarantine Bureau, Qingdao 266001, China；
3. Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao 266071, China

Received:2008-12-09 Revised:2009-07-03 Online:2009-11-15 Published:2010-12-29
Contact: MU Zhi-chun E-mail:muzhichun2000@yahoo.com.cn

Abstract

Abstract:

A high performance liquid chromatography (HPLC) method was developed to identify salmon aquacultured with synthetic astaxanthin through determining relative ratio of chiral isomers of astaxanthin in salmon. Astaxanthin was extracted from flesh of salmon, passed through a Sep-Pak silica gel column for purification, and then analyzed by HPLC. The chromatographic conditions were Pirkle covalent L-leucine column (4.6 mm×25 cm, 5μm) as the stationary phase, hexane-tetrahydrofuran- 2-propanol-triethylamine (77:17:3:3, V/V) as mobile phase with flow rate of 0.75 ml/min, and detection wavelength at 474 nm. Distribution of chiral isomers of astaxanthin in wild salmon and salmon fed with natural feedstuff was similar. However, it revealed a significant difference in salmon fed with synthetic astaxanthin.

Key words: astaxanthin, chiral isomer, salmon, HPLC

CLC Number:

TS254.7

MU Zhi-chun1，ZHANG Ming2，ZHANG Yi-bing1，GUAN En-ping1，LU Peng1，LENG Kai-liang3，DUAN Xiao-juan1，LIU Lin1，ZHENG Shi-xiang1. Rapid Identification of Salmon Aquacultured with Synthetic Astaxanthin through High Performance Liquid Chromatography[J]. FOOD SCIENCE, 2009, 30(22): 318-320.

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Rapid Identification of Salmon Aquacultured with Synthetic Astaxanthin through High Performance Liquid Chromatography

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