Abstract:

Wheat germ protein was hydrolyzed with immoblized alkaline protease in an effort to prepare peptides with antihypertensive effect. The alkaline proteases were immoblized through binding to hollow globular chitosan with glutaraldehyde. Then the immobilized alkaline protease were characterized and the hydrolysis condition of wheat germ protein with it were optimized. Results showed that after 1.0 mg of alkaline proteases were immobilized with 1.5% glutaraldehyde, 90% of the enzyme activity was remained, with the optimum temperature 65℃ and the optimum pH 9.5. The ratio of hydrolysis with the immobilized enzyme for 10 min was 16.5%, and antihypertensive peptides yield was 15.6% when the ratio of enzyme to wheat germ protein was 1:5.

Key words: antihypertensive peptides, wheat germ protein, immobilized alkaline protease