Response Surface Methodology for Optimization of Hydrolysis Conditions for Preparing Antihypertensive Peptides Sichuan Pepper (Zanthoxylum bungeanum) Seed Protein

doi:10.7506/spkx1002-6630-201421035

Abstract

Abstract:

Response surface methodology was used to optimize the hydrolysis conditions of Sichuan pepper (Zanthoxylum
bungeanum) seed protein to prepare antihypertensive peptides. Papain was found to be the best enzyme for the enzymatic
preparation of antihypertensive peptides with angiotensin converting enzyme (ACE) inhibitory activity. The ACE inhibitory
activity of enzymatic hydrolysates of Sichuan pepper seed protein was investigated as a function of hydrolysis time, enzyme
dosage, temperature and pH. The results indicated that the obtained regression model represented the functional relationship
well, and the optimal hydrolysis conditions were found as follows: substrate concentration, 3 g/100 mL; hydrolysis time, 4.9 h;
enzyme dosage, 10 200 U/g; hydrolysis temperature, 37.4 ℃; and pH, 6.9, resulting in an ACE inhibitory rate of 68.00%.

Key words: Sichuan pepper seed protein, angiotensin converting enzyme, antihypertensive peptides, response surface methodology

CLC Number:

TS201.1

WU Hong-yang, JIANG Tai-ling, HU Hui-ming, YANG Cheng-zhi, ZHANG Zhi-qing. Response Surface Methodology for Optimization of Hydrolysis Conditions for Preparing Antihypertensive Peptides Sichuan Pepper (Zanthoxylum bungeanum) Seed Protein[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201421035.

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Response Surface Methodology for Optimization of Hydrolysis Conditions for Preparing Antihypertensive Peptides Sichuan Pepper (Zanthoxylum bungeanum) Seed Protein

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