Extraction of Flagellin from Listeria monocytogenes

doi:10.7506/spkx1002-6300-200923059

Abstract

Abstract:

In this study, the extraction of flagellin from Listeria monocytogenes was studied. Listeria monocytogenes IVb was cultured at 23 ℃ and 37 ℃, respectively. Stronger flagellin-producing capability was observed at 23 ℃ of culture condition. Flagellin was purified through ultra-centrifugation and ammonia sulfate precipitation after acid hydrolysis treatment of Listeria monocytogenes. Results from SDS-PAGE and electronic microscope revealed that ultra-centrifugation combined ammonia sulfate precipitation was an applicable method for flagellin extraction because this simple and time-saving method could provide flagellin with high purity and high yield.

Key words: Listeria monocytogenes, acid hydrolysis, flagellin, ultra-centrifugation

CLC Number:

TS207.3

CHEN Gang，LAI Wei-hua*，DUAN Xia. Extraction of Flagellin from Listeria monocytogenes[J]. FOOD SCIENCE, 2009, 30(23 ): 262-265 https://doi.org/10.7506/spkx1002-6300-200923059

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Extraction of Flagellin from Listeria monocytogenes

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