FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (23): 352-355.doi: 10.7506/spkx1002-6300-200923080

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Antimicrobial Activity of Nisin against Lactic Acid Bacteria

HE Song,GONG Fang-hong,ZHANG De-chun*,GUO Ya-nan   

  1. Department of Pathogenic Biology, Molecular Medicine and Tumor Research Center, Chongqing Medical University, Chongqing
    400016, China
  • Received:2008-12-19 Revised:2009-07-25 Online:2009-12-01 Published:2010-12-29
  • Contact: ZHANG De-chun E-mail:zhangdechun46@sohu.com

Abstract:

Nisin is a bacteriocin used against food spoilage bacteria. The effect of these important antibacterial agents on the growth of lactic acid bacteria was investigated. nisin MIC values were measured by test-tube dilution and disc diffusion methods. The growth curve of lactic acid bacteria was obtained based on its OD600nm absorptions. The results showed that nisin possessed strong and persistent antimicrobial effect on common lactic acid bacteria in dairy industry except for some Lactococcus lactis strains. This study provided the theoretical basis for investigating the MIC and inhibiting time of Nisin to select food-grade recombinant Lactobacillus.

Key words: nisin, lactic acid bacterium, antimicrobial activity, food-grade vector

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