Quality Assessment of Semen Ziziphi Spinosae by HPLC Fingerprinting and Quantitative Analysis

doi:10.7506/spkx1002-6300-201004033

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (4): 143-149.doi: 10.7506/spkx1002-6300-201004033

• Analysis & Detection • Previous Articles Next Articles

Quality Assessment of Semen Ziziphi Spinosae by HPLC Fingerprinting and Quantitative Analysis

LI You1，LIANG Zong-suo1,2,*，ZHOU Zi-yun1,2，YANG Dong-feng1,2

1. College of Life Sciences, Northwest A ＆ F University, Yangling 712100, China；
2. Shaanxi Research Center in Traditional Chinese Medicine Fingerprint and Natural Product Library, Yangling 712100, China

Received:2009-05-08 Revised:2009-11-09 Online:2010-02-15 Published:2010-12-29
Contact: LIANG Zong-suo E-mail:liangzs@ms.iswc.ac.cn

Abstract

Abstract:

This study was aimed to establish a HPLC fingerprinting approach for the effective quality assessment of Semen Ziziphi Spinosae. In HPLC analysis, the chromatographic separation was achieved on an UltimateTM C18 column (250 mm×4.6 mm, 5 μm) with a mobile phase composed of acetonitrile and ultrapure wate at a flow rate of 1.0 mL/min. In addition, detection wavelength and column temperature were set at 210 nm and 30 ℃, respectively. Vector cosine and correlation coefficient methods were used to calculate similarities of 17 samples of Semen Ziziphi Spinosae and cluster analysis was made for them. Results showed that 10 common fingerprint peaks were identified in the samples by HPLC-ELSD while HPLC-UV detection gave 12 common fingerprint peaks. Cluster analysis revealed that the samples were classified into 3 main categories: geographic origins for superior, medium and inferior quality. Samples of Semen Ziziphi Spinosae with superior quality all exhibited a similarity higher than 0.9239 and a jujuboside A content range of 0.8123 to 1.1109 mg/g. It can be concluded that HPLC fingerprinting combined with quantitative analysis of indicator components can provide experimental data for the quality assessment of Semen Ziziphi Spinosae.

Key words: Semen Ziziphi Spinosae, HPLC, fingerprint, ELSD, jujuboside A

CLC Number:

R 282.7

LI You1，LIANG Zong-suo1,2,*，ZHOU Zi-yun1,2，YANG Dong-feng1,2. Quality Assessment of Semen Ziziphi Spinosae by HPLC Fingerprinting and Quantitative Analysis[J]. FOOD SCIENCE, 2010, 31(4): 143-149.

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Quality Assessment of Semen Ziziphi Spinosae by HPLC Fingerprinting and Quantitative Analysis

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