Abstract:

In the present study, a specific monoclonal antibody against Listeria monocytogenes (Lm) was prepared to develop a rapid immune colloidal gold strip for detecting Lm in foods. The developed colloidal gold strip had high specificity, no crossreactivity with L.ivanovii, L.innocua, Escherichia coli, Salmonella, Bacillus subtilis, Staphylococcus aureus and Streptococcus, a sensitivity of 3.9×105 CFU/mL in detecting Lm pure culture and simulated samples of tap water, milk, raw meat products, vegetables, frozen shrimp and bread, and more than 120-day expiration date at 4 ℃. The merits of rapidity, sensitivity, and specificity demonstrate a good applicability of this strip to the detection of Lm in foods.

Key words: Listeria monocytogenes, monoclonal antibody, colloidal gold strip, detection