Abstract:

A HPLC method was developed for the determination of ursolic acid in leaves of Sinojackia sarcocarpa L.Q.Luo. The chromatographic separation was achieved on a Shim-pack VP-ODS C18 column using a mobile phase composed of H2O and H3PO4 (850:147:3, V/V) at a flow rate of 1 mL/min, and the detection wavelength was set at 210 nm. A good linearity of the standard curve for peak area versus ursolic acid concentration was obtained over the range of 0.124－2.48μg/μL (R2 = 0.9971). The average spike recovery of ursolic acid at three levels was 100.5% (n = 3), with 0.91% RSD. In sample preparation, ultrasonic extraction resulted in higher efficiency when compared to reflux extraction and percolation extraction. This method reveals the characteristics of good stability and repeatability, simplicity, and easy operation.

Key words: HPLC, Sinojackia sarcocarpa, ursolic acid