FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (5): 150-154.doi: 10.7506/spkx1002-6300-201005034

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Effects of Temperature and pH on Kinetic Parameters of Enzymatic Hydrolysis of Bovine Bone Protein

CAI Li-hua,MA Mei-hu*   

  1. Meat Science Institute, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2009-05-31 Revised:2009-11-27 Online:2010-03-01 Published:2010-12-29
  • Contact: MA Mei-hu* E-mail:mameihuhn@yahoo.com.cn

Abstract:

In this study, kinetic properties of bovine bone protein hydrolysis by alcalase, neutrase, pepsin and trypsin were investigated. The initial rate of enzymatic hydrolysis was used to evaluate the optimal reaction conditions for these proteases. Results indicated that the optimal temperature and pH were 55 ℃ and pH 11.7 for alcalase, 45 ℃ and pH 7.3 for neutrase, 30 ℃ and pH 1.5 for pepsin, 55 ℃ and pH 9.3 for trypsin. The decreasing order of these proteases for Km and Vmax was neutrase, alcalase, trypsin and pepsin; and pepsin, alcalase, trypsin and neutrase, respectively.

Key words: bone protein, protease, kinetics

CLC Number: