Pulsed-field Gel Electrophoresis Types and Drug Resistance of Enterobacter sakazakii

doi:10.7506/spkx1002-6300-201007045

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (7): 205-209.doi: 10.7506/spkx1002-6300-201007045

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Pulsed-field Gel Electrophoresis Types and Drug Resistance of Enterobacter sakazakii

XU Long-yan1，YUAN Mu-yun1，LIU Jing-yu1，ZHAO Gui-ming2，ZOU Zhi-fei1，YANG Jing1，JIAO Hong1,*

1. Food Laboratory of Technology Center, Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China；
2. Food Safety Institute, Chinese Academy of Inspection and Quarantine, Beijing 100025, China

Received:2009-06-18 Revised:2009-12-08 Online:2010-04-01 Published:2010-12-29
Contact: JIAO Hong1 E-mail:jiaoh@yahoo.com.cn

Abstract

Abstract:

Objectives: To investigate molecular types and drug resistance patterns of Enterobacter sakazakii isolated from foods from Beijing, Xinjiang, Guangdong, and Liaoning or imported foods from New Zealand, USA, and India. Methods: E. sakazakii chromosomal DNA was digested by restriction endonuclease Xba I and analyzed by pulsed-field gel electrophoresis (PFGE). PFGE patterns of E. sakazakii strains from different areas were compared using BioNumberics software to analyze the similarity among strains. Antibiotic susceptibility test was carried out using VITEK2 to analyze drug resistance patterns. Results: Totally 38 E. sakazakii strains revealed 37 PFGE patterns with similarity ranged from 25% to 100%. Neither significantly predominant PFGE patterns nor area specificity were observed. Moreover, some food samples were contaminated by genetically related E. sakazakii clonal strains. Furthermore, the antibiotic susceptibility test also exhibited 9 drug resistance patterns including resistance to furans, cephalosporins, and β-lactam antibiotics. Conclusion: The PFGE method described above may be used for molecular typing and source tracing of E. sakazakii.

Key words: Enterobacter sakazakii, pulsed-field gel electrophoresis, molecular typing, food, drug resistance

CLC Number:

Q939

XU Long-yan1，YUAN Mu-yun1，LIU Jing-yu1，ZHAO Gui-ming2，ZOU Zhi-fei1，YANG Jing1，JIAO Hong1,*. Pulsed-field Gel Electrophoresis Types and Drug Resistance of Enterobacter sakazakii[J]. FOOD SCIENCE, 2010, 31(7): 205-209.

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Pulsed-field Gel Electrophoresis Types and Drug Resistance of Enterobacter sakazakii

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