Capillary Electrophoresis Based Competitive Immunoassay for Chloramphenicol in Animal-derived Food

doi:10.7506/spkx1002-6300-201008031

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (8): 146-149.doi: 10.7506/spkx1002-6300-201008031

• Analysis & Detection • Previous Articles Next Articles

Capillary Electrophoresis Based Competitive Immunoassay for Chloramphenicol in Animal-derived Food

ZHANG Can1,2，MA Hai-le2，WANG Hui2，ZHOU Fan-kun2，WANG Shuo1,*

1. Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Engineering and Biotechnology, Tianjin
University of Science and Technology, Tianjin 300457, China；2. School of Food and Biological Engineering, Jiangsu University,
Zhenjiang 212013, China

Received:2009-07-15 Revised:2009-11-27 Online:2010-04-15 Published:2010-12-29
Contact: WANG Shuo1 E-mail:s.wang@tust.edu.cn

Abstract

Abstract:

A rapid and sensitive approach to performing competitive immunoassay for chloramphenicol in animal-derived food using capillary electrophoresis (CE) with laser-induced fluorescence (LIF) detection was presented. The assay exhibited a linear range of 0.008－5μg/L and a limit of detection of 0.0016μg/L. The sensitivity of this assay was almost 20 times higher than that of the solid-phase ELISA assay and the time consumption was shortened. In the real sample analysis, a detectable level of 0.035μg/kg was obtained.

Key words: chloramphenicol, competitive immunoassay based capillary electrophoresis with a laser-induced fluorescence detector (CEIA-LIF), solid-phase ELISA assay, rapidity, sensitivity, limit of detection

CLC Number:

ZHANG Can1,2，MA Hai-le2，WANG Hui2，ZHOU Fan-kun2，WANG Shuo1,*. Capillary Electrophoresis Based Competitive Immunoassay for Chloramphenicol in Animal-derived Food[J]. FOOD SCIENCE, 2010, 31(8): 146-149.

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Capillary Electrophoresis Based Competitive Immunoassay for Chloramphenicol in Animal-derived Food

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