Study on Content Determination and Antibacterial Effects of Triterpenes from Sclerotia of Poria cocos

doi:10.7506/spkx1002-6630-200901022

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (1): 95-98.doi: 10.7506/spkx1002-6630-200901022

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Study on Content Determination and Antibacterial Effects of Triterpenes from Sclerotia of Poria cocos

SHEN Si，LI Fu-jie， MEI Guang-ming，HUANG Wen

(1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China)

Received:2008-01-02 Revised:2008-05-24 Online:2009-01-01 Published:2010-12-29
Contact: SHEN Si E-mail:shendianbiao@126.com

Abstract

Abstract:

The content of triterpenes extracted from sclerotia of Poria cocos was determined by colorimetry with vanillin-glacial acetic acid and perchloric acid, and the in vitro antibacterial activity of triterpenes was tested by plate growth rate method. The positive results of chemical reactions indicated that methanol extract of sclerotia of Poria cocos contains triterpenes and the content is 1.58%. Its lowest antibacterial concentrations are 0.47, 0.47, 0.95 μg/ml against Escherichia coli, Staphylococcus aureusm, and Pseudomonas aeruginosa respectively. The results also showed that the inhibitory activities of this sclerotia extract against Escherichia coli and Staphylococcus aureusm are relatively stable and hardly affected by time. In conclusion, the triterpenes extracted from sclerotia of Poria cocos have good antimicrobial activity and could be further developed as drug of treating suppurative infection and natural cosmetic of dispelling blain.

Key words: sclerotia of Poria cocos, triterpenes, antibacterial activity

CLC Number:

R284

SHEN Si，LI Fu-jie， MEI Guang-ming，HUANG Wen. Study on Content Determination and Antibacterial Effects of Triterpenes from Sclerotia of Poria cocos[J]. FOOD SCIENCE, 2009, 30(1): 95-98.

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Study on Content Determination and Antibacterial Effects of Triterpenes from Sclerotia of Poria cocos

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