FOOD SCIENCE ›› 2020, Vol. 41 ›› Issue (23): 83-90.doi: 10.7506/spkx1002-6630-20191112-157

• Basic Research • Previous Articles     Next Articles

Antioxidant and Antibacterial Activities of β-Acid Homologue Mixtures with Different Ratios from Hops

YANG Jingwen, LIU Zechang, CHEN Pei, DU Weidong, FAN Xiaolan, SHI Ming, LIU Yumei   

  1. (School of Chemical Engineering and Technology, Xinjiang University, ürümqi 830046, China)
  • Online:2020-12-15 Published:2020-12-28

Abstract: Hops soft resin contains three homologues of β-acids: co-, n- and ad-lupulone, and the ratio among these homologues varies depending on hops varieties. In this experiment, five β-acid homologue mixtures were obtained from the crude β-acid from hops by crystallization, and their antioxidant activity in vitro were investigated comparatively by measuring the inhibitory activity against xanthine oxidase, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and OH radical scavenging activity, total antioxidant activity and inhibition zone diameter. The results showed that the antioxidant activity of these β-acid homologue mixtures were significantly different. Among them, the 1:4 mixture of co-lupulone with n-lupulone + ad-lupulone possessed the strongest antioxidant activity, indicating that the antioxidant activity of n-lupulone + ad-lupulone was stronger than that of co-lupulone. At high concentrations (≥ 40.00 mg/L), mixtures with higher proportion of co-lupulone had stronger antibacterial activity. The antibacterial activity of all samples except the 1:4 mixture of co-lupulone with n-lupulone + ad-lupulone at a concentration of 160.00 mg/L was similar to or even stronger than that of amoxicillin. The sample with the highest proportion of n + ad-lupulone showed the lowest minimum inhibitory concentration (5.00 mg/L) against Staphylococcus aureus. In addition, although the minimum inhibitory concentrations of all samples against Escherichia coli were equally 10.00 mg/L, this one exhibited a bigger diameter of inhibition zone.

Key words: hops; soft resin; β-acid homologues; antioxidant activity; antibacterial activity

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