Study on Separation and Purification of Total Alkaloids from Fritillaria ussuriensis Maxim by Silica Gel Column Chromatography

doi:10.7506/spkx1002-6630-200901028

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (1 ): 119-121.doi: 10.7506/spkx1002-6630-200901028

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Study on Separation and Purification of Total Alkaloids from Fritillaria ussuriensis Maxim by Silica Gel Column Chromatography

ZHAI Ai-hua1，WANG Li-jie2，ZHANG Dong-jie1，LU Ming-ming3

(1. College of Food Science, Heilongjiang August First Land Reclamation University, Daqing 163319, China；
2. College of Animal Husbandry and Veterinary Medicine, Jinzhou Medical College, Jinzhou 121001, China；
3. Dalian Fusheng Pharmaceutical Co. Ltd, Dalian 116600, China)

Received:2008-04-06 Online:2009-01-01 Published:2010-12-29
Contact: ZHAI Ai-hua1 E-mail:zhaiaihua@sina.com

Abstract

Abstract:

Silica gel column chromatography was used to separate and purify total alkaloids from Fritillaria ussuriensis Maxim. The solid phase was thin layer chromatography silica gel G and the mobile phase was ethyl acetate-methanol-ammonia (17:2:1). The purities of the separated products, pemine and peinine were determined using HPLC-ELSD. They are 93.19% and 92.09%, respectively. In conclusion, the separation method of silica gel column chromatography applied in this experiment can be used to gain highly purified products.

Key words: total alkaloids from Fritillaria ussuriensis Maxim, silica gel column chromatography, separation and purification

CLC Number:

TQ464.4

ZHAI Ai-hua1，WANG Li-jie2，ZHANG Dong-jie1，LU Ming-ming3. Study on Separation and Purification of Total Alkaloids from Fritillaria ussuriensis Maxim by Silica Gel Column Chromatography[J]. FOOD SCIENCE, 2009, 30(1 ): 119-121.

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Study on Separation and Purification of Total Alkaloids from Fritillaria ussuriensis Maxim by Silica Gel Column Chromatography

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