FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (1 ): 217-221.doi: 10.7506/spkx1002-6630-200901052
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MIN Wei-hong,CUI Xue-xiang,FANG Li,LIU Jing-sheng
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Abstract:
This article mainly using feedback inhibition of glucose feeding, modulating pH value of fermentation broth, as well as alkalinity neutral and fed- batch culture compound culture to study the high-density culture of the Lactobacillus Bulgaricus. The research indicated, alkalinity neutral and fed-batch culture compound culture conditions as follows: when Lactobacillus Bulgaricus cultivated for 18h or 26h, supplementing batch fluid 5ml in culture fluid the carbon nitrogen ratio is 0.67, and adjust pH value to 6.5 every 1.5h , after raising 36h, the highest thalline concentration of Lactobacillus Bulgaricus is 9.02×1011 CFU/ ml, dry weight of thalline is 14.266 g/L.
Key words: Lactobacillus Bulgarian, alkalinity neutral, fed-batch culture, high-density culture
CLC Number:
Q93.335
MIN Wei-hong,CUI Xue-xiang,FANG Li,LIU Jing-sheng. High-density Cultivation of Lactobacillus Bulgarian in Alkalinity Neutral Combined with Feed-batch[J]. FOOD SCIENCE, 2009, 30(1 ): 217-221.
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URL: https://www.spkx.net.cn/EN/10.7506/spkx1002-6630-200901052
https://www.spkx.net.cn/EN/Y2009/V30/I1 /217