Preparation of Ciprofloxacin Antiserum and Its Determination by Enzyme-linked Immunosorbent Assay (ELISA)

doi:10.7506/spkx1002-6630-200902032

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (2 ): 154-158.doi: 10.7506/spkx1002-6630-200902032

Previous Articles Next Articles

Preparation of Ciprofloxacin Antiserum and Its Determination by Enzyme-linked Immunosorbent Assay (ELISA)

ZENG Hua-jin1， YANG Ran2， QU Ling-bo2,3,*， LI Fei-fei2， LI Jian-jun2

(1. College of Pharmaceutical, Zhengzhou University, Zhengzhou 450001, China ；2. Department of Chemistry, Zhengzhou
University, Zhengzhou 450052, China ；3. Industry University of Henan, Zhengzhou 450001, China)

Received:2007-12-07 Revised:2008-09-17 Online:2009-01-15 Published:2010-12-29
Contact: ZENG Hua-jin1 E-mail:sunny-zhj@163.com

Abstract

Abstract:

Immunogen ciprofloxacin-bovine serum albumin (BSA) and coating antigen ciprofloxacin-ovum albumin (OVA) were prepared by a carbodiimide method and a succinic anhydride method, respectively. The polyclonal antiserum was produced from big ear rabbits immunized with conjugates ciprofloxacin-BSA. As a reagent, the antiserum was also determined with indirect ELISA method and the working range of this method extended from 0.05μg/ml to 10μg /ml (r=0.998). In a comparison of the assay results obtained by ELISA and high performance liquid chromatography (HPLC), there is a good correlation between these two methods.

Key words: ciprofloxacin, antiserum, ELISA

CLC Number:

TS207.7

ZENG Hua-jin1， YANG Ran2， QU Ling-bo2,3,*， LI Fei-fei2， LI Jian-jun2. Preparation of Ciprofloxacin Antiserum and Its Determination by Enzyme-linked Immunosorbent Assay (ELISA)[J]. FOOD SCIENCE, 2009, 30(2 ): 154-158.

[1]	WANG Xiaorui, QIU Hongling, WANG Shouli, ZHU Haihua, ZHOU Li, PING Yang, TAN Jing, WANG Yong, WANG Hui. Preparation of Monoclonal Antibodies against Outer Membrane Protein K of Vibrio parahaemolyticus and Development of a Double Antibody Sandwich ELISA [J]. FOOD SCIENCE, 2021, 42(4): 319-325.
[2]	YAO Tianqi, LAO Cuiyu, WANG Shifeng, HU Guiping, ZHANG Shiwei. Establishment of Indirect Competitive-Enzyme Linked Immunosorbent Assay to Detect Glucocorticoids: Preparation of Broad-Spectrum Monoclonal Antibody [J]. FOOD SCIENCE, 2019, 40(14): 186-191.
[3]	LIU Yao, WEI Qianni, WANG Hong, YANG Jinyi, SUN Yuanming, XU Zhenlin, SHEN Xing, LI Xiangmei, LEI Hongtao. Determination of Chloramphenicol Residue Honey by Indirect Competitive ELISA [J]. FOOD SCIENCE, 2018, 39(16): 336-342.
[4]	YANG Yi, MENG Kunjie, ZHAO Hongqiong, Mikeremu·SHAYIBUZHATI, ZHANG Xiaoying,. ELISA Determination of Enrofloxacin [J]. FOOD SCIENCE, 2017, 38(8): 239-243.
[5]	XIE Manman, LI Jianwu, WANG Guangbin, ZENG Haijuan, ZHAI Xuzhao, DING Chengchao, LIU Wukang, LIU Qing. Preparation and Immunological Characteristics of Monoclonal Antibodies against Vibrio parahaemolyticus [J]. FOOD SCIENCE, 2017, 38(8): 63-68.
[6]	PENG Hongwei, BAI Ruiying, CHEN Dian, SUN Yuanming, XU Zhenlin, ZENG Daoping, LI Duan, WU Zhiquan, YANG Jinyi,. Optimization of Derivatization Conditions by Response Surface Methodology for the Determination of Semicarbazide in Animal-Derived Foods by Enzyme-Linked Immunosorbent Assay [J]. FOOD SCIENCE, 2017, 38(6): 287-294.
[7]	WANG Jing, MA Ningning, SONG Yang. Preparation of Artificial Antigen and Polyclonal Antibody for the Detection of Heterocyclic Amines [J]. FOOD SCIENCE, 2017, 38(4): 45-50.
[8]	WANG Jing, WANG Yao, WANG Fangyu, DENG Ruiguang, HU Xiaofei, YU Qiuying, HAO Junfang, XING Yunrui, HOU Yuze. Preparation of Artificial Antigen and ELISA Identification of Rabbit Polyclonal Antiserum for the Detection of Glyphosate [J]. FOOD SCIENCE, 2017, 38(4): 65-69.
[9]	ZHAI Xuzhao, ZENG Haijuan, WANG Guangbin, DONG Qingli, XIE Manman, DING Chengchao, LIU Wukang, WANG Shujuan, LI Jie, LIU Qing. Preparation of Monoclonal Antibodies against Cronobacter sakazakii and Development of an Indirect Competitive ELISA for detection of this bacterium [J]. FOOD SCIENCE, 2017, 38(20): 268-272.
[10]	ZHANG Can, CUI Hanyu, HAN Yufeng, WANG Xianping, HE Ming, CHEN Bo, LIU Yuan. Development of Immunoagnetic Bead-Based ELISA for the Detection of Carbaryl [J]. FOOD SCIENCE, 2017, 38(2): 296-300.
[11]	ZHAO Yongjuan, WENG Ling, YAN Longjie, LIU Mengqi, ZHANG Lingjing, CAO Minjie,. Quantification of Added Egg White in Surimi Products by Competitive Enzyme Linked-Immunosorbent Assay [J]. FOOD SCIENCE, 2017, 38(14): 284-289.
[12]	XIE Xueqin, LIU Zhou. Recent Advances in Application of Immunological Methods in Detection of Foodborne Vibiro parahaemolyticus [J]. FOOD SCIENCE, 2017, 38(13): 299-305.
[13]	LI Xiaoyu1, ZHANG Chunyu1, GUO Dongquan1, ZHANG Linlin2, YOU Qing3, DONG Yingshan1, WANG Yongzhi1,, LI Qiyun1,. Double-Antibody Sandwich ELISA for the Detection of Transgenic Bar Gene Herbicide-Tolerant Soybean [J]. FOOD SCIENCE, 2016, 37(4): 222-225.
[14]	CHEN Yinnan, CHEN Hua, SHI Xianai, YE Xiaojun, FAN Haiping. Preparation and Application of Monoclonal Antibody against Furazolidone [J]. FOOD SCIENCE, 2016, 37(3): 151-157.
[15]	SONG Chunmei, ZHU Zhenghui, LI Jianwu, LIU Qing. Development of a Test Kit for Rapid Detection of Enterobacter sakazakii Contamination in Milk Powder [J]. FOOD SCIENCE, 2016, 37(24): 233-238.

Preparation of Ciprofloxacin Antiserum and Its Determination by Enzyme-linked Immunosorbent Assay (ELISA)

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments