Abstract:

In this study, xylitol was simply extracted by pure water, and the fat was removed by n-hexane. Then the analysis was performed with a Sugar-PakTM 1 column at 90 ℃ and a mobile phase of pure water, and detected by refractive index detector. The results showed that the average recoveries in cake and juice are 97.0% with a RSD 4.3% and 100.1% with a RSD 2.4%, respectively, and the detection limit (S/N=3) is 50 mg/L. The method is simple, accurate and suitable for the routine determination of xylitol in foods.

Key words: high performance ion exchange chromatography (HPIEC), food, xylitol