Abstract:

Mycobacterium tuberculosis is the pathogenic microorganism of tuberculosis, and it could be infected by contaminated food. A piezoelectric bulk acoustic wave biosensor was used for rapid detection of M. tuberculos attenuated strain H37Ra in food samples. With the pool coefficient of 0.513 cm and the 10-fold diluted culture medium, it was found that the biosensor had a relatively high detection sensitivity, and a linear equation between cell concentrations and wave frequency was also constructed. It was also found that a pre-treatment with 2% NaOH solution could avoid the interference of mixed bacteria but prolong the detection period.

Key words: Mycobacterium tuberculos, piezoelectric bulk acoustic wave biosensor, food detection