FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (16): 258-260.doi: 10.7506/spkx1002-6630-201016057

• Analysis & Detection • Previous Articles     Next Articles

A Colony PCR Assay for Detecting Shiga toxin-producing Escherichia coli

LI Rui1，XU Fang1，ZHANG Zhong-mei1，MIYAMOTO Takahisa2，ZHU Ting-heng3,*   

  1. 1. College of Biological and Pharmaceutical Engineering, Wuhan Polytechnic University, Wuhan 430023, China；
    2. College of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu University, Fukuoka 812-8581, Japan ；
    3. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310000, China
  • Received:2010-03-31 Online:2010-08-25 Published:2010-12-29
  • Contact: ZHU Ting-heng3,* E-mail:thzhu@zjut.edu.cn

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Abstract:

Specific primers targeting for the stx gene of Shiga toxin-producing Escherichia coli (STEC) were designed, and a colony PCR assay method was then developed based on first screening and selective culture in this study. The assay method could specifically identify E. coli O157 carrying stx1 and stx2 genes, while normal E. coli, Bacillus cereus and Staphylococcus aureus could not produce any PCR products. A STEC strain carrying stx1 gene was detected using the assay method in commercial pork and beef products. The method developed in this study is applicable to food samples for the detection of STEC.

Key words: Shiga toxin-producing E. coli, food detection, stx, colony PCR

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