Abstract:

The optimal parameters for the supercritical carbon dioxide (SC-CO2 ) extraction of oat bran oil were determined by orthogonal array design to be: extraction pressure 15 MPa, extraction temperature 35 ℃ and length of extraction 3 hours. The oat bran oil obtained was clear and of golden color and had the characteristic smell of oat. Its fatty acid composition and that of petroleum ether extraction-derived oat bran oil were analyzed by gas chromatography-mass spectrometry (GC-MS), and the results revealed that the major fatty acids found in SC-CO2 extraction-derived oat bran oil were palmitic acid (17.60%), stearic acid (1.32%), oleic acid (40.15%), linoleic acid (37.55%), linolenic acid (1.89%) and elaidic acid (1.52%) and that these compounds in its petroleum ether extraction-derived counterpart were 15.90%, 1.67%, 38.38%, 41.67%, 1.63%, and 1.32%, respectively. The total contents of unsaturated fatty acids in SC-CO2 extraction-derived oat bran oil and its petroleum ether extraction-derived counterpart were 81.11% and 83.00%, respectively.

Key words: supercritical fluid extraction, petroleum ether extraction, oat bran oil, fatty acid composition, GC-MS