Study on Purification and Chemical Compositions of Tea Polysaccharide (TPS-Ⅱ)with Antioxidant Activity

doi:10.7506/spkx1002-6630-200903003

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (3): 25-28.doi: 10.7506/spkx1002-6630-200903003

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Study on Purification and Chemical Compositions of Tea Polysaccharide (TPS-Ⅱ)with Antioxidant Activity

PAN Jian1，CHEN Yan1,2,*，FANG Wei2，SUN Yu-jun3

(1.Engineering Research Center of Bio-process, Ministry of Education, Hefei University of Technology, Hefei 230009, China ；
2.School of Life Science, Anhui University, Hefei 230039, China ；
3.School of Life Science, Anhui Science and Technology University, Fengyang 233100, China)

Received:2008-03-04 Revised:2008-08-04 Online:2009-02-01 Published:2010-12-29
Contact: CHEN Yan1,2,*， E-mail:chenyan600@163.com;jcsw@ahu.edu.cn

Abstract

Abstract:

The tea polysaccharids (TPS)-Ⅱ with antioxidant activity was isolated from the water extract of Liu'an roasted green tea by chromatography. The compositions of TPS-II were identified by electrophoresis and FPLC (fast protein liquid chromatography). The molecular weight of TPS-Ⅱ is 1.01×105 D, and its contents of total saccharide, uronic acid and protein are 85.3%, 28.0% and 2.8% respectively. GC analysis showed that TPS- Ⅱ is composed of L-Rha, L-Fuc, L-Ara, D-Xyl, DMan, D-Glc and D-Gal in molar ratio of 2.98:1.58:4.27:1.00:1.82:2.25:6.98. The IR spectrum of TPS-Ⅱ also presents the peak of polysaccharide absorption characteristics. Furthermore, β-elimination reaction demonstrated that the polysaccharide in TPS- Ⅱ is linked to peptide chains via non-O-glycosidic linkage.

Key words: roasted green tea, tea polysaccharide, purification, monosaccharide composition, infrared spectrum

CLC Number:

S571.1

PAN Jian1，CHEN Yan1,2,*，FANG Wei2，SUN Yu-jun3. Study on Purification and Chemical Compositions of Tea Polysaccharide (TPS-Ⅱ)with Antioxidant Activity[J]. FOOD SCIENCE, 2009, 30(3): 25-28.

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Study on Purification and Chemical Compositions of Tea Polysaccharide (TPS-Ⅱ)with Antioxidant Activity

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