FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (3): 123-126.doi: 10.7506/spkx1002-6630-200903026

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Preliminary Study on Hydroxyl Free Radical Scavenging Activity and Antimicrobial Effect of Fructus Aurantii Immaturus Extracts

LIU Liang1，QI Xiang-yang2，DONG Xu-yan3   

  1. (1.College of Food Science and Technology, Wuhan Polytechnic University, Wuhan 430023, China；
    2.College of Biological and Environmental Science, Zhejiang Wanli University, Ningbo 315100, China；
    3.Institute of Oil Crops Research, Chinese Academy of Agricultural Sciences, Wuhan 430062, China)
  • Received:2008-02-24 Revised:2008-05-27 Online:2009-02-01 Published:2010-12-29
  • Contact: LIU Liang1 E-mail:rll1979@yahoo.com.cn

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Abstract:

The isolation and purification of extracts from Fructus Aurantii Immaturus by-products after alkaloid extraction were studied, and the antioxidant activities and antimicrobial effects of different extracts were also preliminarily explored. The results showed that the optimum separation conditions for limonin by macroporous resin are as follows: sample pH 7.5, mobile solventpH 6.0, and ethanol concentration 70% (V/V). Five fractions (FA1 to FA5) can be successively obtained by eluting Sephadex LH-20 column with 50% and 70% ethanol, and the content of limonin in FA4 is 9.96%. Moreover, the fractions FA1 to FA4 show certain scavenging effects on hydroxyl radical (·OH) and all the five fractions have antimicrobial effects against bacteria.

Key words: Fructus Aurantii Immaturus extracts, antioxidant effects, antimicrobial effects

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