Abstract:

Crude polysaccharide was extracted from whole viscera of Ruditapes philippinarum through enzymolysis, centrifugation for removing protein, ethyl alcohol precipitation and drying. Then inhibitory effects of different concentrations of the polysaccharide against micronucleus formation induced by mitomycin C (MMC) in Vicia faba root tip cells were examined. The results showed that 50, 100 and150 μg/ml of polysaccharide can significantly inhibit micronucleus formation induced by MMC. Furthermore, with the increase of polysaccharide concentration, the inhibitory effect is stronger and the rate of micronucleus is lower.

Key words: Ruditapes philippinarum polysaccharide, mitomycin C, Vicia faba root tip, micronucleus