Abstract:

This study aimed to simply purify mental-binding peptides which were derived from hydrolyzed whey protein by ultra-filtration technology. The main technical parameters were studied, including ultra-filtration pressure, dilution multiple of liquid material, its pH value and temperature, taking membrane flux, peptide recovery rate and initial molar ratio of P to N as evaluation indexes. The results indicated that high-purity and high-activity mental-binding peptides can be obtained a ultrafiltration circle of 45 min with the ultra-filtration membrane of 6000 D molecular weight cut off under the conditions of ultra-filtration pressure 0.125 MPa, dilution multiple of liquid material 4, its pH 5.5 and temperature 45 ℃. Under these conditions, the appearance time of precipitates can be postponed for 15 min, which is created from the reaction between NaH2PO4 and CaCl2. Meanwhile, the peptide recovery rate is 84.10%.

Key words: mental-binding peptides, ultra-filtration technology, optimization