Abstract:

The fusion gene of sCT and CGRP was designed according to the principles of genetic engineering method and the bias codon used by plant. An AMV RNA4 5＇-UTR sequence and a 6×His tag were added before the gene, and the Gly was added at the end of the fusion gene. The fusion gene was obtained from pUC(AMV / CGRP / msCT) by Sac I and Sma I digestion, then cloned into plant expression vector pBI121 by T4 DNA ligase to construct recombinant plasmid pBI AMV / CGRP / msCT, and transformed into E.coli DH5α to be kept. The gene sequencing indicated that the expression vector pBI AMV / CGRP / msCT has been successfully constructed.

Key words: salmon calcitonin, calcitonin gene-related peptide, genetic engineering, fusion expression, hypertension, osteoporosis