Study on Protoplast Preparation and Regeneration of Cordyceps sinensis

doi:10.7506/spkx1002-6630-200905038

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (5): 166-170.doi: 10.7506/spkx1002-6630-200905038

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Study on Protoplast Preparation and Regeneration of Cordyceps sinensis

GUO Cheng-jin，ZHAO Run

(College of Chemistry and Life Science, Tianjin Normal University, Tianjin 300387, China)

Received:2007-12-10 Revised:2008-10-24 Online:2009-03-01 Published:2010-12-29
Contact: GUO Cheng-jin， E-mail:chengjin@vip.sina.com

Abstract

Abstract:

The primary factors affecting protoplast preparation and regeneration of Cordyceps sinensis were discussed with orthogonal analysis in this study. The results showed that the optimum preparation and regeneration process is as follows: digesting mycelia, which has been cultured for 3 d with 2.0% lywallzyme and 1.0% snailase at 32 ℃ for 2 h, with 0.6 mol/L KCl and 0.6 mol/L mannitol as osmotic pressure stabilizers for protoplast preparation and regeneration media respectively. Under the above conditions, the protoplast yield and regeneration rate reach 2.98 × 108/ml and 42.09 × 10-2, respectively.

Key words: Cordyceps sinensis, orthogonal analysis, protoplast preparation, regeneration, osmotic pressure stabilizer

CLC Number:

Q949.3

GUO Cheng-jin，ZHAO Run. Study on Protoplast Preparation and Regeneration of Cordyceps sinensis[J]. FOOD SCIENCE, 2009, 30(5): 166-170.

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Study on Protoplast Preparation and Regeneration of Cordyceps sinensis

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