FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (5): 249-252.doi: 10.7506/spkx1002-6630-200905057

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Inhibitory Effects of Almond Protein Hydrolysate against AngiotensinⅠ-converting Enzyme

LIU Ning,QIU Nong-xue*,ZHU Zhen-bao,NIU Peng-fei,WANG Li-yuan,MIAO Li-li   

  1. (College of Food Engineering and Nutritional Science, Shaanxi Normal University, Xi’an 710062, China)
  • Received:2008-04-02 Revised:2008-05-22 Online:2009-03-01 Published:2010-12-29
  • Contact: QIU Nong-xue*, E-mail:nongxueq@snnu.edu.cn

Abstract:

Almond protein was respectively hydrolyzed with Protamex, Alcalase, Neutrase, Flavourzyme, Proleather FG-F and Papain, and then the angiotensin I-converting enzyme (ACE) inhibitories activities of obtained hydrolysates were detected by high performance liquid chromatography (HPLC). With the degree of hydrolysis (DH) and the ACE-inhibitory activity of hydrolysate as evaluation indexes, the hydrolysis course and the digestion stability in vitro of the hydrolysate were studied. The results indicated that Proleather FG-F and Alcalase have better hydrolysis ability to almond protein than other proteases, and the IC50 values of their hydrolysates to ACE are 1.24 mg/ml and 0.98 mg/ml, respectively. Moreover, after simulated gastrointestinal digestion their hydrolysates still have high ACE-inhibitory activity.

Key words: almond protein, ACE-inhibitory activity, degree of hydrolysis, simulated gastrointestinal digestion

CLC Number: