Denaturing High-Performance Liquid Chromatography Coupled with Polymerase Chain Reaction for Detecting seudomonas aeruginosa in Dairy and Cosmetic Products

doi:10.7506/spkx1002-6630-200906029

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (6): 139-142.doi: 10.7506/spkx1002-6630-200906029

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Denaturing High-Performance Liquid Chromatography Coupled with Polymerase Chain Reaction for Detecting seudomonas aeruginosa in Dairy and Cosmetic Products

CAO Ji-juan1，ZENG Qiu-yue1,2，SUN Zhe-ping1，WANG Qiu-yan1，PEI Yi-jun1，ZHAO Xin1

(1. Liaoning Entry-Exit Inspection and Quarantine Bureau, Dalian 116001, China；
2. Shenyang Agricultural University, Shenyang 110161, China)

Received:2008-05-19 Revised:2008-06-30 Online:2009-03-15 Published:2010-12-29
Contact: CAO Ji-juan1 E-mail:cjj0909@163.com

Abstract

Abstract:

Objective: To rapidly identify Pseudomonas aeruginosa using denaturing high-performance liquid chromatography DHPLC) coupled with polymerase chain reaction (PCR). Methods: The primers and probes for specific PCR amplification ere designed based on the reported sequence of exotoxin type A (ETA)gene of P.aeruginosa, and then the specificity, sensitivity nd stability of this method were analyzed. Results: In analysis of Pseudomonas aeruginosa and other species of Pseudomonas y this method, only P.aeruginosa can be detected, and the sensitivity is 100 CFU/ml. Conclusion: The PCR-DHPLC method s rapid, specific, sensitive and relatively simple for the identification of P.aeruginosa.

Key words: DHPLC, seudomonas aeruginosa, exotoxin type A gene, detection

CLC Number:

TS207.4

CAO Ji-juan1，ZENG Qiu-yue1,2，SUN Zhe-ping1，WANG Qiu-yan1，PEI Yi-jun1，ZHAO Xin1. Denaturing High-Performance Liquid Chromatography Coupled with Polymerase Chain Reaction for Detecting seudomonas aeruginosa in Dairy and Cosmetic Products[J]. FOOD SCIENCE, 2009, 30(6): 139-142.

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Denaturing High-Performance Liquid Chromatography Coupled with Polymerase Chain Reaction for Detecting seudomonas aeruginosa in Dairy and Cosmetic Products

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