Determination of Saccharide Components and Contents in Blueberry Juice by HPLC

doi:10.7506/spkx1002-6630-200906053

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (6): 229-231.doi: 10.7506/spkx1002-6630-200906053

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Determination of Saccharide Components and Contents in Blueberry Juice by HPLC

ZHANG Ying-chun，DONG Ai-jun，YANG Xin*，ZHANG Hua，MA Ying

(College of Food Science and Engineering, Harbin Institute of Technology, Harbin 150090, China)

Received:2008-04-21 Revised:2008-07-28 Online:2009-03-15 Published:2010-12-29
Contact: YANG Xin*， E-mail:zyc229@163.com

Abstract

Abstract:

Objective: To set up an examination method for determining saccharides in blueberry juice by HPLC. Method: Saccharides were extracted from samples with water using ultrasonic, and then their components and contents were determined by HPLC. Agilent Zorbax carbohydrate (4.6 × 250 mm, 5 μm) column was adopted, mobile phase was acetonitrile-water (75:25, V:V), and the flow rate was 1 ml/min. Results: The calibration curve of saccharide concentrations is well linear in the range of 0.5 to 10 mg/ml, the correlation coefficient is over 0.9989 (n=5), the RSD is 0.36% to 2.18% (n=5) in precision experiment, and the average recovery rate is 91.36% to 101.34%. Conclusion: This method is convenient, rapid and effective for separation and quantitative analysis of saccharides in blueberry juice.

Key words: blueberry juice, fructose, glucose, sucrose, HPLC

CLC Number:

O652.63

ZHANG Ying-chun，DONG Ai-jun，YANG Xin*，ZHANG Hua，MA Ying. Determination of Saccharide Components and Contents in Blueberry Juice by HPLC[J]. FOOD SCIENCE, 2009, 30(6): 229-231.

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Determination of Saccharide Components and Contents in Blueberry Juice by HPLC

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