FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (8): 171-176.doi: 10.7506/spkx1002-6630-200908035

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Detection of Transgenic Components in Soybean Products by SYBR Green Real-Time PCR

WANG Xiao-hua1 LI Jian-xiang1 WANG Guo-qing2 LI Xin-li1 SHAO Jing-dong3 FU Chun-ling1,*   

  1. (1. College of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China
    2. College of Preclinical Medicine and Life Science, Soochow University, Suzhou 215123, China
    3. Zhangjiagang Entry-Exit Inspection and Quarantine Bureau, Zhangjiagang 215633, China)
  • Received:2008-06-08 Revised:2008-09-04 Online:2009-04-15 Published:2010-12-29
  • Contact: FU Chun-ling1,* E-mail:fuchunling@suda.edu.cn

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Abstract:

A quantitative method to detect the transgenic component in soybean by using real-time PCR technique based on fluorescence dye SYBR Green was investigated in this study. The endogenous lectin gene and transgenic target CP4EPSPS gene were amplified through the design of specific primers, and the transgenic content was then calculated according to the standard curve equation. Meanwhile the specificity of PCR amplification was analyzed with corresponding melting curves .The results showed that the standard curves of lectin and CP4EPSPS genes have good linear relationship, and their R2 values are 0.9984 and 0.9953, respectively. Moreover, the recovery rate of the method is between 95% and 110% and the detection limit is 0.01%. In conclusion, this method is fast, sensitive, simple, accurate, specific and of high throughput, and can be used to quantificationally detect transgenic components in soybean products. 

Key words: real-time quantitative PCR, fluorescence dye SYBR Green I, transgenic soybean

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