FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (8): 218-220.doi: 10.7506/spkx1002-6630-200908048

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Determination of Trehalose Content of Grifola frondosa Mycelia by Anthrone-Sulfuric Acid Method

LI Yan-ling SHI Ren-jiu ZHANG Xian-zhong MIAO Miao   

  1. (Department of Biological Sciences, Taishan Medical College, Taian 271016, China)
  • Received:2008-06-07 Revised:2008-09-04 Online:2009-04-15 Published:2010-12-29
  • Contact: LI Yan-ling E-mail:lingl816@163.com

Abstract:

Abstract: The procedure of colorimetric analysis of mycelium trehalose of Grifola frondosa was investigated in the present study. The results showed that when Grifola frondosa mycelium sample solution is added with anthrone-sulfuric acid reagent at half of its volume, heated at 100 ℃ for 5 min and then analyzed at 625 nm, the trehalose content in Grifola frondosa mycelium is determined as 3.88 mg/ml, and the average recovery is 106.5%. Also, the stability and repeatability of this method are good. In conclusion, this method is convenient, rapid, accurate and suitable for the determination of rehalose content in Grifola frondosa mycelium.

Key words: Grifola frondosa, trehalose, anthrone-sulfuric acid method

CLC Number: