Purification and Structural Analysis of Acidic Polysaccharide from Hedysarum radix

doi:10.7506/spkx1002-6630-200909016

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (9): 70-72.doi: 10.7506/spkx1002-6630-200909016

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Purification and Structural Analysis of Acidic Polysaccharide from Hedysarum radix

MA Jun-shou XIONG Shuang-li* JIN Hong

College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang 621010, China

Received:2008-07-14 Online:2009-05-01 Published:2010-12-29
Contact: XIONG Shuang-li E-mail:xiongshuangli@swust.edu.cn

Abstract

Abstract:

Crude polysaccharide was prepared from Hedysarum radix in Gangu of Gansu province by firstly aqueous extraction and then ethanol precipitation. An acidic polysaccharide was purified from the crude polysaccharide extract by DEAE-Sepharose Fast Flow ion exchange column chromatography followed by Sepharose 6B Fast Flow gel column chromatography and then was subjected to composition and structural analysis. Agarose gel electrophoresis pattern indicated that the acidic polysaccharides is of high purity and further was proved to be heterogeneous by ultraviolet spectroscopy and infrared spectroscopy. Thin-layer chromatogram showed that the monosaccharide compositions are xylose, glucuronic acid or galacturonic acid, galactosamine hydrochloride or galactosamine hydrochloride, glucuronolactone or galactonolactone with the presence of sulfate group.

Key words: Hedysarum radix, polysaccharides, isolation and purification, structural analysis

CLC Number:

TS202.1

MA Jun-shou XIONG Shuang-li* JIN Hong. Purification and Structural Analysis of Acidic Polysaccharide from Hedysarum radix[J]. FOOD SCIENCE, 2009, 30(9): 70-72.

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Purification and Structural Analysis of Acidic Polysaccharide from Hedysarum radix

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