High Cell Density Fed-batch Cultivation of Rhizopus oryzae As3.819 for Production of L-lactic Acid

doi:10.7506/spkx1002-6630-200909031

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (9): 133-136.doi: 10.7506/spkx1002-6630-200909031

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High Cell Density Fed-batch Cultivation of Rhizopus oryzae As3.819 for Production of L-lactic Acid

PAN Li-jun LIU Jing JIANG Shao-tong ZHENG Zhi WANG Ying

College of Biology and Food Engineering, Hefei University of Technology, Hefei 230009, China

Received:2008-08-04 Revised:2009-01-08 Online:2009-05-01 Published:2010-12-29
Contact: PAN Li-jun E-mail:panlijun1955@163.com

Abstract

Abstract:

Production of L-lactic acid was investigated by high density fed-batch fermentation of Rhizopus oryzae As3.819. The optimum culture conditions in shaking flask were as follows: inoculation size 5%, inoculation age 24 h, medium volume in flask 40% and mass glucose to ammonium sulfate ratio 20:1. Higher density of Rhizopus oryzae As3.819 cell was obtained through glucose feedback feeding method in a 15-L fermentor, and the cell dry weight was up to 18.45 g/L. The L-lactic acid yield reached a stable level, 2.25 g/L·h after 5 repeated semi-continuous fermentations, which was higher 82.9% than that of batch fermentation, and the cell dry weight was increased by 92.9% .

Key words: high density fed-batch cultivation, feedback feeding, Rhizopus oryzae, L-lactic acid

CLC Number:

Q815

PAN Li-jun LIU Jing JIANG Shao-tong ZHENG Zhi WANG Ying. High Cell Density Fed-batch Cultivation of Rhizopus oryzae As3.819 for Production of L-lactic Acid[J]. FOOD SCIENCE, 2009, 30(9): 133-136.

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High Cell Density Fed-batch Cultivation of Rhizopus oryzae As3.819 for Production of L-lactic Acid

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