Microbial Cholesterol Oxidase: sources, physical properties and applications

doi:10.7506/spkx1002-6630-200909055

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (9): 225-229.doi: 10.7506/spkx1002-6630-200909055

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Microbial Cholesterol Oxidase: sources, physical properties and applications

ZHANG Ling YANG Hai-lin SUN Yan HUO Hui-zhi WANG Wu*

Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi 214122, China

Received:2008-07-24 Revised:2008-11-25 Online:2009-05-01 Published:2010-12-29
Contact: WANG Wu E-mail:wangwu@jiangnan.edu.cn

Abstract

Abstract:

Recently, microbial cholesterol oxidase (COD) has drawn too much attention and lots of related studies have been done due to their perfect application values in clinical diagnosis, food processing, biopharmaceutical industry, and gene engineering, etc. This article reviewed the recent research progress on COD from the following aspects: (1) variety of available bacterial sources, (2) enzymatic properties, (3) molecular structure basis, (4) methodology in COD activity assay, (5) COD gene cloning, and (6) applications of COD in clinical diagnosis, food processing, biopharmaceutical industry, molecular biological study and disinsection.

Key words: cholesterol oxidase, enzymatic properties, structure, application, gene engineering

CLC Number:

ZHANG Ling YANG Hai-lin SUN Yan HUO Hui-zhi WANG Wu*. Microbial Cholesterol Oxidase: sources, physical properties and applications[J]. FOOD SCIENCE, 2009, 30(9): 225-229.

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