Abstract:

Protein was extracted from Xanthoceras sorbifolia Bunge. kernel by using reversed micelles. Single-factor test combined with quadratic general rotary design was applied to study the effects of factors such as surfactant concentration, ion concentration, pH value, oil to water ratio and temperature. The optimal conditions of protein extraction from Xanthoceras sorbifolia Bunge. kernel by reversed micelles were determined as follows: cetyl trimethyl ammonium bromide (CTAB) concentration 0.03 mol/L, pH 10, oil to water ratio 1:1 and temperature 30 ℃. This optimal operating condition resulted in the high protein yield of 56.27%. In addition, a regression mathematical model was established, which could predict the extraction yield of protein from Xanthoceras sorbifolia Bunge. kernel accurately.

Key words: reversed micelles, Xanthoceras sorbifolia Bunge. kernel protein, forward extraction yield