FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (10): 131-134.doi: 10.7506/spkx1002-6630-200910025

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Study on Determination and Extraction of Total Polyphenols from Leaves of Punica granatum L.

QIU Tao-tao1,WANG Hua2,*,MAO shi-hong1   

  1. (1. College of Food Science, Southwest University, Chongqing 400716, China;
    2. Citrus Research Institute, Chinese Academy of Agricultural Sciences, Chongqing 400712, China)
  • Received:2008-11-07 Revised:2009-01-18 Online:2009-05-15 Published:2010-12-29
  • Contact: WANG Hua2,*, E-mail:wanghua40·126.com

Abstract:

Folin-Ciocalteu method was used to determine the content of total polyphenols from leaves of Punica granatum L.. The effect of extraction conditions on the extraction rate of total polyphenols from extracts was evaluated by single factor and orthogonal tests. The optimal extraction conditions of total polyphenols from leaves of Punica granatum L. are as follows: 75% alcohol as extraction solvent, temperature 70 ℃, extraction time1.5 h, material to liquid ratio 1:20 and 1 mm of particle size. Under these conditions, the maximum extraction rate of total polyphenols from leaves of Punica granatum L. was 16.63%.

Key words: leaves of Punica granatum L., total polyphenols, Folin-Ciocalteu, extraction

CLC Number: