FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (10): 140-143.doi: 10.7506/spkx1002-6630-200910027

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Analysis of Syringin Content in New Resource Acanthopanax.sessiliflorus (Rupr.et Maxim.) Seem and Other Seven Species of Acanthopanax Miq. by RP-HPLC

LI Li-li1，ZHENG Li-sheng1，LIU Xiang-qian1,*，ZHANG Xiao-dan1，OH Se-wook2   

  1. (1.Department of Pharmaceutical Engineering, Central South University, Changsha 410083, China；
    2. Korea Food and Drug Administration, Seoul 122704, Korea)
  • Received:2008-07-17 Revised:2008-10-13 Online:2009-05-15 Published:2010-12-29
  • Contact: LIU Xiang-qian1,*， E-mail:lxq0001cn@163.com

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Abstract:

The objective of this study was to determine the contents of syringin in 8 species of Acanthopanax Miq. such as A. sessiliflorus (Rupr. et Maxim.) Seem, A. leucorrhizus (Oliv.) Harms var. fulvescens Harms et Rehd., Acanthopanax giraldii Harms, A. senticosus (Rupr. et Maxin) Harms, A. divaricatus var. albeofructus Yook, A. koreanum Nakai, A. gracilistylus W.W. Smith, and A.trifoliatus (L.) Merr by RP-HPLC. The column, mobile phase, flow rate, detection RP-HPLC were ODS C18 as An orthogonal experimental design was employed to optimize the extraction conditions of syringin. The method displayed good linearity within the concentration range of 0.0148 to 0.074 mg/ml (r = 0.9999) and a regression equation of Y = 15234560 X－ 7 881.767 (R2 = 0.9999) was obtained. The average spike recovery was 98.24%. Acanthopanax leucorrhizus var.fulvescens Harms et Rehd. Had the highest content of syringin among the 8 species of Acanthopanax Miq., followed by A.sessiliflorus (Rupr. et Maxim.) Seem. The optimum extraction process of syringin was adding material to 15 times volume water and then boiling the water for 3.5 h. Our results suggested the simplicity and high reliability of this method to analyze syringin.

Key words: A. sessiliflorus (Rupr. et Maxim.) Seem, Acanhopanax Miq. , RP-HPLC, syringin

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