FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (11): 197-201.doi: 10.7506/spkx1002-6630-200911044

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Study on Viable but Non-culturable State of Two Species of Health-indicator Bacteria in Bottled Water

CAO Xiao-hong,YANG Zheng*,LU Mei-fang,HAN Chao-long,WANG Xiao-ye   

  1. (Tianjin Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology, Tianjin University of Science
    and Technology, Tianjin 300457, China)
  • Received:2008-09-27 Revised:2009-01-08 Online:2009-06-01 Published:2010-12-29
  • Contact: YANG Zheng*, E-mail:yangzheng1681@163.com

Abstract:

Escherichia coli is generally used as the health-indicator bacterium in microorganism detection of bottled water. However, when induced by low temperature or Cu2 +, Escherichia coli can enter the viable but non-culturable state and not be detected by the general detecting culture medium. Staphylococcus aureus, a gram-positive pathogenic bacterium, is also found the existence of viable but non-culturable state. Therefore, these bacteria in this state will be escaped only using the present microorganism detecting way. In the resuscitation experiment of the present study, it was found that catalase and Tween 20 could excite the culturable ability of the two species of bacteria from the viable but non-culturable state in a short time.

Key words: viable but non-culturable state, bottled water, Escherichia coli, Staphylococcus aureus, catalase

CLC Number: