FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (13): 52-55.doi: 10.7506/spkx1002-6630-200913011

Previous Articles     Next Articles

Antimicrobial Activity of Extracts from 5 Kinds of Gourd Vegetables

GUO Qi1,WEI Yu-xi1,*,YIN Bang-zhong2,GUO Dao-sen1,LIU Lei1   

  1. 1.Department of Biology, Qingdao University, Qingdao 266071, China;
    2.Yellow Sea Fisheries Research Institute, China Academy of Fishery Sciences, Qingdao 266071, China
  • Received:2008-11-18 Revised:2008-12-08 Online:2009-07-01 Published:2010-12-29
  • Contact: WEI Yu-xi E-mail:yuxiw729@163.com

Abstract:

The paper disc diffusion method was applied to determine the antimicrobial activities of petroleum ether, ethyl acetate, 95% ethanol and water extracts and crude polysaccharides from 5 kinds of gourd vegetables including pumpkin, bitter gourd, cucumber, sponge gourd and wax gourd against Escherichia coli and Staphylococcus aureus. The results showed that the ethyl acetate extract from pumpkin displayed the highest antimicrobial activity. The extract was separated by silica gel chromatography and thin-layer chromatography (TLC) and two fractions with higher antibacterial activity were obtained, which exhibited broad-spectrum resistance to Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Shigella, Tetracoccus and Pseuodomonas fluorescens, especially to Staphylococcus aureus. Their minimal inhibition concentrations (MICs) against Staphylococcus aureus were 1.25 mg/ml and 2.5 mg/ml, respectively.

Key words: gourd vegetables, antimicrobial activity, pumpkin, column chromatography

CLC Number: