Optimization of Medium Components for Biomass and Arachidonic Acid Production by Mortierella alpina ME-1 Using Response Surface Methodology

doi:10.7506/spkx1002-6630-200913048

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (13): 205-211.doi: 10.7506/spkx1002-6630-200913048

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Optimization of Medium Components for Biomass and Arachidonic Acid Production by Mortierella alpina ME-1 Using Response Surface Methodology

PENG Chao1，HUANG He1,2,*，JIN Ming-jie1，XIAO Ai-hua2，YU Wen-tao1,2，LIU Xin1

1. College of Life Science and Pharmacy, Nanjing University of Technology, Nanjing 210009, China；
2.Jiangsu Provincial Innovation Center for Industrial Biotechnology, Nanjing Univetsity of Technology, Nanjing 211816, China

Received:2008-08-04 Revised:2008-10-25 Online:2009-07-01 Published:2010-12-29
Contact: PENG Chao1， E-mail:pengsuper_2004@163.com

Abstract

Abstract:

The media for biomass and arachidonic acid (ARA) production by Mortierella alpina ME-1 were optimized in shake flask cultures using response surface methodology (RSM). Two imperial polynomial models were developed, and tae maximum biomass yield of 36.86 g/L that appeared at glucose, yeast extract (YE), KH2PO4 and NaNO3 of 90.16, 12.50, 3.80 and 3.54 g/L, respectively, while a maximum ARA yield of 9.65 g/L that appeared at glucose, YE, KH2PO4 and NaNO3 of 103.16, 11.66, 3.80 and 3.43 g/L , respectively, in 6.5-day fermentation were predicted. Verified by validation experiments showed excellent correlations between their predicted and measured values. The results of bioreactor fermentation also illustrated that the optimized culture medium enhanced both biomass (34.21 ± 1.01 g/L in 5-d fermentation) and ARA (9.86 ± 0.45 g/L in 6-d fermentation) production by M. alpine ME-1 in a large-scale fermentation process.

Key words: arachidonic acid, M. alpina, optimization, response surface methodology, fermentation, lipids

CLC Number:

TS201.3

PENG Chao1，HUANG He1,2,*，JIN Ming-jie1，XIAO Ai-hua2，YU Wen-tao1,2，LIU Xin1. Optimization of Medium Components for Biomass and Arachidonic Acid Production by Mortierella alpina ME-1 Using Response Surface Methodology[J]. FOOD SCIENCE, 2009, 30(13): 205-211.

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Optimization of Medium Components for Biomass and Arachidonic Acid Production by Mortierella alpina ME-1 Using Response Surface Methodology

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