Abstract:

Cellulase-catalyzed hydrolysis was used to extract polysaccharides from the collected mycelia of Gloeostereum incarnatum 105 after submerged fermentation. The extraction technology was optimized by orthogonal factorial design based on single factor test. The results showed that the optimal conditions for extracting polysaccharides from mycelia of Gloeostereum incarnatum 105 were as follows: solid/liquid ratio 1:60, pH 5.3, cellulose amount 2.5%, and hydrolysis temperature 43 ℃ for a hydrolysis duration of 2.0 h; under these conditions, the yield of polysaccharides reached 4.86%.

Key words: Gloeostereum insarnatum, polysaccharides, enzymatic hydrolysis, cellulase