Abstract:

Compared to traditional column chromatography, high speed countercurrent chromatography (HSCCC) has many advantages such as rapid separation, low sample adsorption, good separation reproducibility, etc. Therefore, this study employed HSCCC to purify crude flavonoid extract from Rosa roxburghii Tratt fruit. The purification was performed using a twophase solvent system composed of chloroform, methanol and water (4:4:2, V/V) at a flow rate of 1.0 ml/min, when the rotation speed and the detection wavelength were set at 800 r/min and 254 nm, respectively. The results showed that five flavonoid compounds whose purities were 75.6%, 95.1%, 73.5%, 89.1% and 87.7%, respectively were obtained, and their masses were 21, 8, 11, 20 and 55 mg, respectively (obtained from 318 mg of dry crude flavonoid extract from Rosa roxburghii Tratt fruit).

Key words: high speed countercurrent chromatography (HSCCC), Rosa Roxburghii Tratt, flavonoid