Abstract:

A HPLC method was developed for determining melamine in milk and dairy products. The analysis was carried out using the Waters HPLC system equipped with a C18 column (150 mm × 4. 6 mm, 5 μm) and a diode array detector set at 235.8 nm. The mobile phase consisted of 0.202% haptane sodium sulphate acetonitrile solution and 0.210% citric acid aqueous solution (10:90, V/V) with a flow rate of 1.0 ml/min, and the column temperature was set at 30 ℃. A good linear correlation (the regression equation: A = 59746C + 183.48) between the peak area and the melamine determination was found in the range between 0.1 and 1.6 μg/ml with a correlation coefficient of 0.9973. The recoveries for melamine in three spiked dairy products ranged 98.3%－ 99.2% with a RSD of less than 1.52 %.

Key words: milk, dairy products, melamine, HPLC