Abstract:

The selenium tolerance of Bifidobacterium animalis 01 isolated from feces of long-life persons living in Bama area of Guangxi in China was investigated and the conditions for selenium enrichment in this strain were optimized by single-factor and orthogonal array methods. The maximum concentration of selenium in the culture medium was 12 μg/ml for Bifidobacterium animalis 01. The optimal conditions for selenium enrichment in Bifidobacterium animalis 01 were determined as follows: Se(Ⅳ) concentration 8 μg/ml, inoculation amount 5%, adding selenium to the medium at the 6th h of culture, and culture time 48 h. Under these conditions, the selenium content in Bifidobacterium animalis 01reached (898 ± 74) μg/g and the conversion rate of selenium was (28.1 ± 2.4)%.

Key words: Bifidobacterium animalis 01, selenium tolerance, selenium enrichment