HPLC Determination of Nucleosides in Different Tissues of Xylaria nigripes

doi:10.7506/spkx1002-6630-200916049

Abstract

Abstract:

Objective: To determine the kinds and contents of nucleosides in different tissues (natural sclerotium, natural stroma, cultivated stroma and fermented mycelium) of Xylaria nigripes. Methods: Samples were extracted using water in an ultrasonic field, and the extracts were filtrated through 0.45 μm membrane prior to HPLC analysis. The chromatographic separation was achieved on a YWG C18 column (4.0 mm × 300 mm，10 μm) at 40 ℃ using a mobile phase consisting of a mixture of methanol and water (0 min: 0% → 8 min: 10% → 10 min:15%) at a flow rate of 1 ml/min, and the analytes were detected at 259 nm. Results: The natural sclerotium, natural stroma, cultivated stroma and fermented mycelium of Xylaria nigripes mainly contained uridine and adenosine, and the content of nucleosides in sclerotium was higher than that in stroma and fermented mycelium. Besides, the content of nucleosides in natural stroma was similar to that in cultivated stroma. Conclusion: nucleosides were rich in Xylaria nigripes, and the cultivated stroma can replace the natural stroma and sclerotium.

Key words: Xylaria nigripes, uridine, adenosine, HPLC

CLC Number:

TS201.2

LIAO Qiong，ZHOU Rong，CHEN Zuo-hong*. HPLC Determination of Nucleosides in Different Tissues of Xylaria nigripes[J]. FOOD SCIENCE, 2009, 30(16): 228-230.

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HPLC Determination of Nucleosides in Different Tissues of Xylaria nigripes

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