Abstract:

Objective: To explore and evaluate the regulatory effects of IgG from bovine colostrum on cytokine network in gastrointestinal mucoca immunity system. Methods: Rabbit serum was collected 3, 2, and 2 h after oral administration, intraperitoneal injection and ear intravenous injection with IGF-1, respectively, to examine the contents of IFN-γ, TNF-α, TGF-β1, IL-1β, IL-10, IL-4, IL-6 and IL-1Ra by ELISA assay. Results: After oral administration, the contents of inflammatory cytokines like IFN-γ, IL-6, IL-1 and TNF-α were significantly increased while those of anti-inflammatory cytokines like TGF-β1, IL-10, IL-4 and IL-1Ra were significantly decreased. The IgG administration by intraperitoneal injection or ear intravenous injection resulted in the content increases of the anti-inflammatory cytokines and the content decrease of the inflammatory cytokines. Conclusions: IgG which is administered orally can not be absorbed but identified easily by intestinal epithelial cells, macrophages, mast cells, NK cells and other innate immune cells and thus induces the production of inflammatory cytokines and inhibits the production of anti-inflammatory cytokines. In contrast, IgG administered in the ways of intraperitoneal injection and intravenous injection is easy to bind with the inhibitory FcγR Ⅱ B of immune cells so as to inhibit the production of inflammatory cytokines and induce the production of anti-inflammatory cytokines.

Key words: bovine colostrum, IgG, cytokine, gastrointestinal mucosa immunity system