Comparison of Ion Chromatographic and Barium Sulfate Turbidimetric Determination of Sulfate Group in Agarose

doi:10.7506/spkx1002-6630-200918079

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (18 ): 340-343.doi: 10.7506/spkx1002-6630-200918079

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Comparison of Ion Chromatographic and Barium Sulfate Turbidimetric Determination of Sulfate Group in Agarose

YANG Xian-qing1，ZHAO Yong-qiang1,2，QI Bo1，LI Lai-hao1,*，CHEN Sheng-jun1，CEN Jian-wei1

1. South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China；
2. College of Food Science and Technology, Guangdong Ocean University, Zhanjiang 524025, China

Received:2009-06-02 Online:2009-09-15 Published:2010-12-29
Contact: YANG Xian-qing E-mail:yxqgd@163.com

Abstract

Abstract:

Agarose was dry ashed and the ash was separately diluted with ultrapure water and 1.0 mol/L hydrochloric acid to volume for measuring sulfate group content by ion chromatography (IC) and barium sulfate turbidimetry (BST). The IC method exhibited a good linear relationship in the SO42- concentration range of 1.0－15.0 mg/L, with a correlation coefficient of 0.9996, the RSD of 6 repetitions was 1.40%, indicating a high precision, and the recoveries in at two spiked levels were from 86.33% to 97.40%; As for the BST method, the linear range was 6.0－30.0 mg/L and the correlation coefficient was 0.9991, the RSD of 6 repetitions was 3.51%, and the recoveries in at two spiked levels were from 84.17% to 96.67%.These results show that both IC and BST can provide an accurate determination of sulfate group in agarose.

Key words: agarose, sulfate group, ion chromatography, barium sulfate turbidimetry

CLC Number:

YANG Xian-qing1，ZHAO Yong-qiang1,2，QI Bo1，LI Lai-hao1,*，CHEN Sheng-jun1，CEN Jian-wei1. Comparison of Ion Chromatographic and Barium Sulfate Turbidimetric Determination of Sulfate Group in Agarose[J]. FOOD SCIENCE, 2009, 30(18 ): 340-343.

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Comparison of Ion Chromatographic and Barium Sulfate Turbidimetric Determination of Sulfate Group in Agarose

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