Abstract:

Aspergillus oryzae was selected as the initial strain for breeding cellulase-producing mutants. A mutant strain, C3 was obtained through combinatorial mutagenization with UV and DES. The optimal mutation conditions were sequential treatment with 0.1 ml of 15% DES and then illumination with 30 W UV lamp for 60 seconds at a distance of 20 cm. The cellulase activity of this high-yield strain reached 100.23 U/100 ml, which was 1.39 times of that of initial strain.

Key words: Asperqillus oryzae, breeding, cellulase